Cell clustering using pixel clusters (frequencies): Gaussian blur sigma=2, pixel clusters hClusters using z-score cap=3 and iter thresh=2, normalize by size, k=20

1 Heatmaps of pixel clusters x markers

2 Compare pixel clusering with cell clustering

3 Cell-based FlowSOM clustering using pixel clusters

3.1 Cell cluster x frequency of pixel clusters

3.2 Cell cluster x marker

4 New pixel clusters using information from cell clusters